Introduction: Individuals with Autism Spectrum Disorder may present behavioral, communication and social interaction changes, in addition to the presence of impairments in sensory processing, such as auditory hypersensitivity. Objective: to describe the creation and validation of an auditory behavior test to evaluate auditory hypersensitivity in Wistar rats that were exposed to valproic acid during the uterine period as an experimental model for autism. Methodology: A Box was built, divided into two parts: one acoustically coated and the other not. To build the Box, we used: 10 mm thick MDF wooden plates, 50X50 cm acoustic foam plates 2 cm thick, nails, glue, acrylic plate. The Box was 50 cm high, 80 cm wide and 55 cm long. As acoustic stimulus, non-verbal sounds from a digital band were used at frequencies of 0.5, 1, 2 and 4KHz (drum, afoxé, castanets and reco-reco), through a sound box located inside the non-acoustically coated part. at two different sound intensities. Results: At frequencies of 2KHz and 4KHz, there was a statistically significant difference in the area of the Box without acoustic covering and in the corridor in the control group in relation to the group with autism. The animals entered the corridor and acoustically protected areas more often in search of hearing protection and anxiety. Conclusion: The creation and validation of the auditory behavior test to evaluate auditory hypersensitivity in autistic Wistar rats was effective, since it presented a statistically significant difference at frequencies of 2KHz and 4KHz.