Resumen de Duplex One-Step RT-PCR for the detection of Dengue and Zika viruses

Daniela Cristina Sensato Monteiro, Louhanna Pinheiro Rodrigues Teixeira, Natália Vasconcelos de Souza, Léo Santiago, Leonardo Tondello Martins, Kaio Cesar Simiano Tavares, Danielle Malta Lima

• Dengue and Zika, arboviruses are common in Brazil and present with similar clinical symptoms making specific diagnoses difficult. Therefore, a Duplex One-step RT-PCR method for dengue and zika virus was developed and validated in vitro and in silico, based on the dengue's NS1 region and zika's Envelope region.