Efectos subletales asociados a la resistencia a indoxacarb en la palomilla dorso de diamante

• Autores: José Francisco Rodríguez Rodríguez, Ernesto Cerna Chávez, Yisa María Ochoa Fuentes, Sarahyt Santamaría González-Figueroa, Luis Patricio Guevara Acevedo, Hugo César Cisneros López
• Localización: Ecosistemas y Recursos Agropecuarios, ISSN-e 2007-901X, ISSN 2007-9028, Vol. 11, Nº. 1, 2024
• Idioma: español
• DOI: 10.19136/era.a11n1.3888
• Títulos paralelos:
  • Sublethal effects associated with resistance to indoxacarb in diamondback moth
• Enlaces
  • Texto completo (pdf)
• Resumen
  • español

    Plutella xylostellaes una plaga de las más importantes en los cultivos de brassicaceasalrededor del mundo. El indoxacarb se utiliza ampliamente para el control de larvas de lepidópteros plaga. Este insecticida presenta una actividad lenta y efectos residuales prolongados los cuales impactan los hábitosy la fisiología de las plagas. Como objetivo se estableció la evaluación del efecto de concentraciones subletales de indoxacarb en la biología y tiempo de desarrollo de la palomilla dorso de diamante. Se realizó presión de selección del insecticida indoxacarb sobre P. xylostella, aplicando laCL20de la generación anterior por las siguientes diez generaciones, se calculó la CL20de la línea resistente (G10) y posteriormente se aplicó esta concentración a larvas de L3. Se evaluó el efecto en el porcentaje y peso de pupas, emergencia de adultos y oviposición para dos generaciones; además, del tiempo de desarrollo de huevo, larva, pupa y adulto. Los resultadosmostraron que la resistencia y la exposición a CL20de indoxacarb provocaron cambios en la biología y el desarrollo de P. xyllostella. Estose manifestó en un aumento del porcentaje y peso de pupas formadas y emergencia de adultos, junto con una prolongación del tiempo de desarrollo de más de 10 días. Sin embargo, se observó una disminución de más del 50% en el número de huevos por hembra.

  • English

    Plutella xylostellais one of the most important pests of brassicaceous crops around the world. Indoxacarb is widely used to control lepidopteran pest larvae. This insecticide has slow activity and prolonged residual effects which impact the habits and physiology of pests. The objective was to evaluate the effect of sublethal concentrations of indoxacarb on the biology and development time of the diamondback moth. Selection pressure of the insecticide indoxacarb was carried out on P. xylostella, applying the LC20of the previous generation for the next ten generations, the LC20of the resistant line (G10) was calculated and this concentration was subsequently applied to L3 larvae. The effect on the percentage and weight of pupae, adult emergence and oviposition for two generations was evaluated; in addition, the development time of egg, larva, pupa and adult. The results showed that resistance and exposure to LC20of indoxacarb caused changes in the biology and development of P. xyllostella. This was manifested in an increase in the percentage and weight of formed pupae and emergence of adults, along with a prolongation of development time of more than 10 days. However, a decrease of more than 50% in the number of eggs per female was observed.

• Referencias bibliográficas
  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. Journal Economic Entomology 18: 265-267.
  • Andaloro JT, Wing KD, Green J, Lang EB (2000) StewardTM dispersion and cotton leaf interactions: impact on cotton insect pests and safety...
  • Mota-Sanchez, D. and J.C. Wise. (2023) The Arthropod Pesticide Resistance Database. Michigan State University. http://www.pesticideresistance.org....
  • Bashari E, Ghadamyari M, Sendi JJ (2014) Toxicity, and biological and biochemical effects of hexaflumuron on the elm leaf beetle, Xanthogaleruca...
  • Bauer M, Greenwood SJ, Clark KF, Jackman P, Fairchild W (2013) Analysis of gene expression in Homarus americanus larvae exposed to sublethal...
  • Desneux N, Decourtye A, Delpuech, JM (2007) The sublethal effects of pesticides on beneficial arthropods. Annual Review of Entomology 52:...
  • Finney DJ (1971). Probit Analysis. 3rd Edition. Cambridge at the University Press. Cambridge, Reino Unido 120 p.
  • Guedes RNC, Smagghe G, Stark JD, Desneux, N (2016) Pesticide-induced stress in arthropod pests for optimized integrated pest management programs....
  • Han W, Zhang S, Shen F, Liu M, Ren C, Gao, X (2012) Residual toxicity and sublethal effects of chlorantraniliprole on Plutella xylostella...
  • Harshman L.G, Song, KD, Casas J, Schuurmans A, Kuwano E, Kachman SD, et al. (2010) Bioassays of compounds with potential juvenoid activity...
  • Kang WJ, Koo HN, Jeong DH, Kim HK, Kim J, Kim GH (2017) Functional and genetic characteristics of chlorantraniliprole resistance in the diamondback...
  • Li Z, Feng X, Liu SS, You, M Furlong, MJ (2016) Biology, ecology, and management of the diamondback moth in China. Annual Review of Entomology...
  • Liu, J. L., Yang, X., Zhang, H. M., Chen, X., & Wu, J. C. (2013). Effects of indoxacarb on total protein, RNA, and DNA contents in the...
  • Mahmoudvand M, Abbasipour H, Garjan AS, Bandani, AR (2011) Sublethal effects of indoxacarb on the diamondback moth, Plutella xylostella (L.)...
  • Marak RM, Firake DM, Sontakke PP, Behere GT (2017) Mode of inheritance of Indoxacarb resistance in diamondback moth, Plutella xylostella (L.)...
  • Nehare S, Moharil MP, Ghodki BS, Lande GK, Bisane KD, Thakare AS, et al. (2010) Biochemical analysis and synergistic suppression of indoxacarb...
  • Pu X, Yang Y, Wu S, Wu Y (2010) Characterisation of abamectin resistance in a field‐evolved multiresistant population of Plutella xylostella....
  • Rehan A, Freed S (2015) Fitness cost of methoxyfenozide and the effects of its sublethal doses on development, reproduction, and survival...
  • Shen JL, Tan JG, Xiao B, Tan FJ, You ZP (1991) Monitoring and forecasting of Helicoverpa armigera (Lepidoptera: Noctuidae) resistance to pyrethroid...
  • Song L, Zhang J (2013) Sublethal effects of indoxacarb and beta-cypermethrin on Plutella xylostella (Lepidoptera: Plutellidae). Acta Entomologica...
  • Sugumaran M (2010) Chemistry of cuticular sclerotization. In Advances in insect Physiology 39:151-209).
  • Tufail M, Takeda M (2008) Molecular characteristics of insect vitellogenins. Journal of Insect Physiology, 54(12): 1447-1458.
  • Verhoeven KJ, Macel M, Wolfe L M, Biere A (2011) Population admixture, biological invasions and the balance between local adaptation and inbreeding...
  • Vojoudi S, Saber M, Gharekhani G, Esfandiari E (2017) Toxicity and sublethal effects of hexaflumuron and indoxacarb on the biological and...
  • Wang G, Huang X, Wei H, Fadamiro HY (2011) Sublethal effects of larval exposure to indoxacarb on reproductive activities of the diamondback...
  • Wang XL, Su W, Zhang JH, Yang YH, Dong K, Wu YD (2016) Two novel sodium channel mutations associated with resistance to indoxacarb and metaflumizone...
  • Wing, K. D., Andaloro, J. T., McCann, S. F., & Salgado, V. L. (2010). Indoxacarb and the sodium channel blocker insecticides: chemistry,...
  • Wing KD, Schnee ME, Sacher M, Connair M (1998) A novel oxadiazine insecticide is bioactivated in lepidopteran larvae. Archives of Insect Biochemistry...
  • Yin C, Wang R, Lu, C, Zhao K, Wu Q, Wang Z, Yang G (2019) Monitoring, cross-resistance, inheritance, and synergism of Plutella xylostella...
  • Yin XH, Wu QJ, Li XF, Zhang YJ, Xu BY (2008) Sublethal effects of spinosad on Plutella xylostella (Lepidoptera: Yponomeutidae). Crop Protection...
  • Zhao JZ, Li YX, Collins HL, Gusukuma-Minuto L, Mau RFL, Thompson GD, et al. (2002) Monitoring and characterization of diamondback moth (Lepidoptera:...