Acceso usuarios
María Lourdes Tapia, Andrea Carrión Elguera, Faustino Félix Beraún Barrantes, Rossana Falcon Ramos
Micropropagation in vitro has played an important role to achieve this breakthrough, the first studies were conducted by Knud-son in 1922 and Morel in the year 1856. Made Micropropagation was 3 rinses with sterile water, immersion in alcohol for one second from mature closed capsule, which I desinfecto with sodium hypochlorite at 1%, and flamed with the lighter, after 30 days are they formed protocormos. Transferred 3 times on MS medium, when the seedlings reach a size of 3-5 cm were accli-mated to using a substrate of White Moss in a greenhouse.
