Asociación de genoma completo para el hábito de crecimiento en trigo harinero (Triticum aestivum L.)

• Ana L. Gómez-Espejo ^[1] ; Carolina P. Sansaloni ^[2] ; Juan Burgueño ^[2] ; Fernando H. Toledo ^[2] ; M. Humberto Reyes-Valdés
  1. [1] Universidad Autónoma Agraria Antonio Narro

     Universidad Autónoma Agraria Antonio Narro

     México

     
  2. [2] Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT)
• Localización: Ecosistemas y Recursos Agropecuarios, ISSN-e 2007-901X, ISSN 2007-9028, Vol. 8, Nº. 2 (Mayo-Agosto), 2021
• Idioma: español
• DOI: 10.19136/era.a8n2.2912
• Títulos paralelos:
  • Genome-wide association for growth habit in bread wheat (Triticum aestivum L.)
• Enlaces
  • Texto completo  1    2  
• Resumen
  • español

    Resumen: El hábito de crecimiento de los trigos harineros (primavera e invierno) está determinado primordialmente por el proceso de vernalización, regulado por los genes Vrn. Algunos estudios sugieren la implicación de otros genes en la diferenciación de dicha característica. Por ello, se tiene por objetivo la identificación genómica de regiones con una posible relación funcional al hábito de crecimiento. Se realizó un estudio de asociación del genoma completo (GWAS) con dos enfoques de análisis: a) FarmCPU (Unificación de Probabilidad Circulante de Modelo fijo y aleatorio) y b) BLINK (Información Bayesiana y Desequilibrio de ligamiento Anidado Iterativamente) implementados en el paquete GAPIT de R. Se utilizaron datos de 25 681 loci de polimorfismo de un solo nucleótido (SNP’s) de 7 757 colectas de T. aestivum del banco de germoplasma del CIMMYT clasificadas en cuanto a su hábito de crecimiento. Se identificaron 593 marcadores SNP’s con una alta asociación al hábito de crecimiento (p < 0.01). Pero solo 70 marcadores formaban bloques de asociación (BA) en 11 cromosomas. Como se esperaba, se identificó un BA cercano al gen Vrn1 en el cromosoma 5A. Adicionalmente se identificaron 20 regiones genómicas (BA) con asociación al hábito de crecimiento, en las que se detectó la presencia de proteínas de respuesta al estrés implicadas en procesos de aclimatación y des-aclimatación. Los resultados sugieren la participación de nuevos genes en la determinación del hábito de crecimiento en Triticum aestivum y su potencial predictivo en colectas no clasificadas.

  • English

    Abstract: The growth habit in bread wheat (spring and winter) is mainly determined by the process of vernalization, regulated by the Vrn genes. Several studies suggest the involvement of other genes in this differential trait. Therefore, the aim of this research was the genomic identification of regions with a possible functional relationship to growth habit. A genome wide association study (GWAS) was performed by using two different approaches: a) FarmCPU (Fixed and random model Circulating Probability Unification) and b) BLINK (Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway), both implemented in the GAPIT package of R. The source was a data set of 25 681 single nucleotide polymorphism loci (SNP’s) from 7 757 accessions of T. aestivum stored in the CIMMYT germplasm bank and classified according to growth habit. A total of 593 SNP’s markers strongly associated to their growth habit was identified (p < 0.01). But only 70 markers formed association blocks (BA) within 11 chromosomes. As expected, at least one block was located close to the Vrn1 gene of the 5A chromosome. Additionally, 20 genomic regions (BA) were identified with putative functional relationship to growth habit, with response stress proteins involved in acclimation and de-acclimation processes. The findings suggest of new genes implicated in the growth habit for Triticum aestivum and its predictive potential for unclassified genetic accessions.

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