Gestational Exposure to Bisphenol A Affects the Function and Proteome Profile of F1 Spermatozoa in Adult Mice

• Autores: Md Saidur Rahman, Woo-Sung Kwon, Polash Chandra Karmakar, Sung-Jae Yoon, Buom-Yong Ryu, Myung-Geol Pang
• Localización: Environmental health perspectives, ISSN 0091-6765, Vol. 125, Nº. 2, 2017, págs. 238-245
• Idioma: inglés
• DOI: 10.1289/EHP378
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• Resumen

  • Maternal exposure to the endocrine disruptor bisphenol A (BPA) has been linked to offspring reproductive abnormalities. However, exactly how BPA affects offspring fertility remains poorly understood.

    The aim of the present study was to evaluate the effects of gestational BPA exposure on sperm function, fertility, and proteome profile of F1 spermatozoa in adult mice.

    Pregnant CD-1 mice (F0) were gavaged with BPA at three different doses (50 μg/kg bw/day, 5 mg/kg bw/day, and 50 mg/kg bw/day) on embryonic days 7 to 14. We investigated the function, fertility, and related processes of F1 spermatozoa at postnatal day 120. We also evaluated protein profiles of F1 spermatozoa to monitor their functional affiliation to disease.

    BPA inhibited sperm count, motility parameters, and intracellular ATP levels in a dose-dependent manner. These effects appeared to be caused by reduced numbers of stage VIII seminiferous epithelial cells in testis and decreased protein kinase A (PKA) activity and tyrosine phosphorylation in spermatozoa. We also found that BPA compromised average litter size. Proteins differentially expressed in spermatozoa from BPA treatment groups are known to play a critical role in ATP generation, oxidative stress response, fertility, and in the pathogenesis of several diseases.

    Our study provides mechanistic support for the hypothesis that gestational exposure to BPA alters sperm function and fertility via down-regulation of tyrosine phosphorylation through a PKA-dependent mechanism. In addition, we anticipate that the BPA-induced changes in the sperm proteome might be partly responsible for the observed effects in spermatozoa.

    Rahman MS, Kwon WS, Karmakar PC, Yoon SJ, Ryu BY, Pang MG. 2017. Gestational exposure to bisphenol-A affects the function and proteome profile of F1 spermatozoa in adult mice. Environ Health Perspect 125:238–245; http://dx.doi.org/10.1289/EHP378

• Referencias bibliográficas
  • Auger, J, Eustache, F, Maceiras, P, Broussard, C, Chafey, P, Lesaffre, C. (2010). Modified expression of several sperm proteins after chronic...
  • Bauer, SM, Roy, A, Emo, J, Chapman, TJ, Georas, SN, Lawrence, BP. (2012). The effects of maternal exposure to bisphenol A on allergic lung...
  • Benninghoff, A. (2007). Toxicoproteomics—the next step in the evolution of environmental biomarkers.. Toxicol Sci. 95. 1-4
  • Bolt, HM, Stewart, JD. (2011). Highlight report: the bisphenol A controversy.. Arch Toxicol. 85. 1491-1492
  • Bush, LA, Herr, JC, Wolkowicz, M, Sherman, NE, Shore, A, Flickinger, CJ. (2002). A novel asparaginase-like protein is a sperm autoantigen...
  • Cao, W, Gerton, GL, Moss, SB. (2006). Proteomic profiling of accessory structures from the mouse sperm flagellum.. Mol Cell Proteomics. 5....
  • Dobrzyńska, MM, Radzikowska, J. (2013). Genotoxicity and reproductive toxicity of bisphenol A and X-ray/bisphenol A combination in male mice.....
  • D’Souza, R, Gill-Sharma, MK, Pathak, S, Kedia, N, Kumar, R, Balasinor, N. (2005). Effect of high intratesticular estrogen on the seminiferous...
  • Emahazion, T, Beskow, A, Gyllensten, U, Brookes, AJ. (1998). Intron based radiation hybrid mapping of 15 complex I genes of the human electron...
  • Hayes, TB, Khoury, V, Narayan, A, Nazir, M, Park, A, Brown, T. (2010). Atrazine induces complete feminization and chemical castration in male...
  • Heindel, JJ, Newbold, R, Schug, TT. (2015). Endocrine disruptors and obesity.. Nat Rev Endocrinol. 11. 653-661
  • Hengstler, JG, Foth, H, Gebel, T, Kramer, PJ, Lilienblum, W, Schweinfurth, H. (2011). Critical evaluation of key evidence on the human health...
  • Huang, B, Jiang, C, Luo, J, Cui, Y, Qin, L, Liu, J. (2014). Maternal exposure to bisphenol A may increase the risks of Parkinson’s disease...
  • Ijiri, TW, Merdiushev, T, Cao, W, Gerton, GL. (2011). Identification and validation of mouse sperm proteins correlated with epididymal maturation.....
  • Ijiri, TW, Vadnais, ML, Huang, AP, Lin, AM, Levin, LR, Buck, J. (2014). Thiol changes during epididymal maturation: a link to flagellar angulation...
  • Imai, H, Suzuki, K, Ishizaka, K, Ichinose, S, Oshima, H, Okayasu, I. (2001). Failure of the expression of phospholipid hydroperoxide glutathione...
  • Inaba, K. (2011). Sperm flagella: comparative and phylogenetic perspectives of protein components.. Mol Hum Reprod. 17. 524-538
  • Jin, P, Wang, X, Chang, F, Bai, Y, Li, Y, Zhou, R. (2013). Low dose bisphenol A impairs spermatogenesis by suppressing reproductive hormone...
  • Kinch, CD, Ibhazehiebo, K, Jeong, JH, Habibi, HR, Kurrasch, DM. (2015). Low-dose exposure to bisphenol A and replacement bisphenol S induces...
  • Kwon, WS, Rahman, MS, Lee, JS, Yoon, SJ, Park, YJ, Pang, MG. (2015). Discovery of predictive biomarkers for litter size in boar spermatozoa.....
  • Liu, C, Duan, W, Zhang, L, Xu, S, Li, R, Chen, C. (2014). Bisphenol A exposure at an environmentally relevant dose induces meiotic abnormalities...
  • Miki, K, Qu, W, Goulding, EH, Willis, WD, Bunch, DO, Strader, LF. (2004). Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic...
  • Mocarelli, P, Gerthoux, PM, Ferrari, E, Patterson, DG, Kieszak, SM, Brambilla, P. (2000). Paternal concentrations of dioxin and sex ratio...
  • Nomura, K, Imai, H, Koumura, T, Arai, M, Nakagawa, Y. (1999). Mitochondrial phospholipid hydroperoxide glutathione peroxidase suppresses apoptosis...
  • Okada, A, Kai, O. (2008). Effects of estradiol-17β and bisphenol A administered chronically to mice throughout pregnancy and lactation on...
  • Phillips, KP, Tanphaichitr, N. (2008). Human exposure to endocrine disrupters and semen quality.. J Toxicol Environ Health B Crit Rev. 11....
  • Rahman, MS, Kwon, WS, Lee, JS, Kim, J, Yoon, SJ, Park, YJ. (2014). Sodium nitroprusside suppresses male fertility in vitro.. Andrology. 2....
  • Rosenfeld, CS, Sieli, PT, Warzak, DA, Ellersieck, MR, Pennington, KA, Roberts, RM. (2013). Maternal exposure to bisphenol A and genistein...
  • Ryan, JJ, Amirova, Z, Carrier, G. (2002). Sex ratios of children of Russian pesticide producers exposed to dioxin.. Environ Health Perspect....
  • Salian, S, Doshi, T, Vanage, G. (2009). Perinatal exposure of rats to bisphenol A affects the fertility of male offspring.. Life Sci. 85....
  • Sikka, SC, Wang, R. (2008). Endocrine disruptors and estrogenic effects on male reproductive axis.. Asian J Androl. 10. 134-145
  • Suarez, SS. (2008). Control of hyperactivation in sperm.. Hum Reprod Update. 14. 647-657
  • Takai, Y, Tsutsumi, O, Ikezuki, Y, Hiroi, H, Osuga, Y, Momoeda, M. (2000). Estrogen receptor-mediated effects of a xenoestrogen, bisphenol...
  • Takai, Y, Tsutsumi, O, Ikezuki, Y, Kamei, Y, Osuga, Y, Yano, T. (2001). Preimplantation exposure to bisphenol A advances postnatal development.....
  • Trasande, L, Zoeller, RT, Hass, U, Kortenkamp, A, Grandjean, P, Myers, JP. (2015). Estimating burden and disease costs of exposure to endocrine-disrupting...
  • Turner, RM, Eriksson, RL, Gerton, GL, Moss, SB. (1999). Relationship between sperm motility and the processing and tyrosine phosphorylation...
  • Visconti, PE. (2009). Understanding the molecular basis of sperm capacitation through kinase design.. Proc Natl Acad Sci U S A. 106. 667-668
  • Yan, L, Liu, J, Wu, S, Zhang, S, Ji, G, Gu, A. (2014). Seminal superoxide dismutase activity and its relationship with semen quality and....