[2]
;
Jhony Navat Enríquez-Vara
[1]
;
Gabriel Rincón-Enríquez
[2]
;
Evangelina Esmeralda Quiñones-Aguilar
[2]
México
El uso de Streptomyceses una estrategia importante para el control de enfermedades postcosecha causadas por Colletotrichum. El objetivo de este estudio fue evaluar la actividad antifúngica in vitro de aislados actinobacterianos contra C. gloeosporioides e identificar aquellos con mayor actividad mediante secuenciación del gen ARNr 16S. Los experimentos se realizaron usando un diseño completamente al azar con tres repeticiones para cada aislado: serie BVEZ (76 aislados) y serie BVEZMW (122 aislados). La identificación molecular se realizó por secuenciación de Sanger usando el gen ARNr 16S. Los resultados mostraron que el 28 y 21% de los aislados exhibieron actividad antifúngica contra C. gloeosporioides para las series BVEZ y BVEZMW, respectivamente. La identificación molecular reveló que los cinco aislados sobresalientes pertenecen al género Streptomyces. Los Streptomycesspp. de este estudio podrían ser una fuente de metabolitos secundarios antifúngicos con potencial para el control de la antracnosis causada por C. gloeosporioides.
The use of Streptomycesis an important strategy for the control of postharvest diseases caused by Colletotrichum. The objective of this study was to evaluate the in vitro antifungal activity of actinobacterial isolates against C. gloeosporioides and identify those with higher activity by sequencing the 16S rRNA gene. The experiments were performed using a completely randomized design with three replications for each isolate: BVEZ series (76 isolates) and serial BVEZMW (122 isolates). Molecular identification was performed by Sanger sequencing using the 16S rRNA gene. The results showed that 28 and 21% of the isolates exhibited antifungal activity against C. gloeosporioides for the BVEZ and BVEZMW series, respectively. Molecular identification revealed that the five outstanding isolates belong to the genus Streptomyces. The Streptomycesspp. in this study could be a source of antifungal secondary metabolites with potential for the control of anthracnose caused by C. gloeosporioides.